Lipidomics Analysis Service—Agilent 1200 HPLC System

Summary

This system is unique in that it has a quaternary pump plus an additional isocratic pump that is used to introduce post-column reagents. Compounds can be first separated, then induced to react with an agent that changes their properties to make them detectable in the Multi-Wavelength Detector (MWD). This HPLC also has a thermostatted autosampler and column compartment. It is used primarily for low-pressure Fast Protein Liquid Chromatography (FPLC) lipoprotein profiling and for HPLC measurement of inositol phosphates including phytate. Like the other HPLCs, it is versatile and useful for other methods as well.