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Liquid Chromatography and LC/MSs—Thermo Scientific EASY-nLC 1200 Nanoflow Liquid Chromatograph

Liquid chromatography (LC) is a technique widely used to separate compounds from samples before analysis and is often used in conjunction with mass spectrometry. We offer you a Thermo Scientific EASY-nLC 1200 Nanoflow Liquid Chromatograph-based liquid chromatography and LC/MS analysis service.

Name of the Instrument/Technicial Service

Thermo Scientific EASY-nLC 1200 Nanoflow Liquid Chromatograph

Introduction

NanoLC Overview: 1. Pressure range: 0-1200 bar 2. Flow range: 20-2000 nL/min (Recommended: 100-1000 nL/min) 3. Samples injected via autosampler 4. Software integration with MS and instrument control using Xcalibur 4.0 5. NanoViper fitting technology enables low dead volume liquid flow path connectivity. Minimizes peak broadening due to mixing. 6. nano-ESI ionization source developed in-house utilizes a 10 µm emitter.

MANUFACTURER: Thermo Fisher Scientific

Summary

The The Thermo Scientific EASY-nLC 1200 is a nanoflow liquid chromatograph capable of precise gradient flow (water/acetonitrile only) from 20 nL/min to 2 µL/min with pressures up to 1200 bar. For LC-MS the EASY-nLC 1200 is coupled to a Thermo Scientific LTQ-XL linear ion trap (LIT) mass spectrometer with nanoelectrospray ionization (nESI) capabilities. The LTQ-XL has MSn capabilities, which is a powerful analytical tool for use in structural analysis. This mass spectrometer can provide molecular weight and sequence information for small peptides (MW below 2000 Da) at the femtomole level with proper analyte introduction.

Technical Capabilities

Important Notes

Sample Preparation: 1. Filtered 2. Degassed

If you are interested in this service, please feel free to contact us. We will provide you with detailed solutions and technical support.

For research use only, not intended for any clinical use.

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