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Mass Spectrometry Analysis Service—Bruker Microflex LRF

Mass spectrometry has become invaluable in a wide range of fields and applications, including proteomics. The development of high throughput and quantitative MS proteomics workflows has expanded the scope of protein structure, function, modifications, and overall protein dynamics. In addition, MS has been widely used in metabolomics and glycomics analysis. Creative Proteomics is equipped with advanced mass spectrometers to support your experiments in academia or industry with the professional mass spectrometry-based analysis described above.

Name of the Instrument/Technicial Service

Bruker Microflex LRF

Introduction

1. Time-of-Flight MS 2. Two Modes of Operation: 1) Reflectron Mode: small molecules, peptides (better resolution) 2) Linear Mode: fragile molecules (oligosaccharides), > 5 kDa 3. Capable of Measuring Negative and Positive Ions 4. 337 nm Nitrogen Laser 5. Mass Range: 1) Reflectron Mode: Up to 20,000 m/z 2) Linear Mode: Up to 500,000 m/z 6. Resolution: 1) Reflectron Mode: > 15,500 for small peptides (i.e. Somatostatin 28 at 3147.5 Da) 2) Linear Mode: > 3,200 for small peptides (i.e. Bombesin at 1619.82 Da) > 1200 for smaller proteins (i.e. Cytochrome C at 12.3 kDa) > 495 for larger proteins (i.e. Protein A at 44.6 kDa) 7. Mass Accuracy: 1) Reflectron Mode: Internal Calibration: < 3 ppm error External Calibration: < 25 ppm error 2) Linear Mode: Internal Calibration: < 45 ppm error External Calibration: < 50 ppm error 8. Limits of Detection: Reflectron Mode: S/N ~ 25 for 1 fmol Glu-Fib (1570.7 Da) Linear Mode: S/N > 130 for 500 fmol BSA (66 kDa)

MANUFACTURER: Bruker

Summary

Analysis of biopolymers (peptides, proteins, DNA, RNA, oligosaccharides), organometallics, synthetic polymers

Creative Proteomics offers you Bruker Microflex LRF-based mass spectrometry services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.

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