Proteomics Laboratory Services
Proteomics Laboratory Services
Mass spectrometry has become invaluable in a wide range of fields and applications, including proteomics. The development of high throughput and quantitative MS proteomics workflows has expanded the scope of protein structure, function, modifications, and overall protein dynamics. In addition, MS has been widely used in metabolomics and glycomics analysis. Creative Proteomics is equipped with advanced mass spectrometers to support your experiments in academia or industry with the professional mass spectrometry-based analysis described above.
System Overview: 1. Optimum mass range 50-2000 m/z, 4000 m/z in limited capacity 2. Resolution of > 1000 across optimum mass range on a full MS scan 3. Zoom and turbo scan features 4. Positive / negative ion modes 5. ESI and APCI sources 6. MSn (i.e. MS-MS-MS-...) mode for structural analysis 7. LC-MS capability 8. Protein identification with SEQUEST software 9. Deconvolution tool for protein mass measurement
MANUFACTURER: Thermo Fisher Scientific
The Finnigan LCQ is a quadrupole field ion trap mass spectrometer with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources. The LCQ has MSn capabilities, which is a powerful analytical tool for use in structural analysis. This mass spectrometer can provide molecular weight and sequence information for small peptides (MW below 2000 Da) at the femtomole level with proper analyte introduction.
Sample Preparation: 1. All samples should be in an ESI-friendly solvent system such as 50/50 Water/Acetonitrile, Water/Methanol, etc. 2. For positive ion samples, a small amount of acetic or formic acid (0.1% v/v) can be added to enhance the signal. TFA is not recommended. 3. For direct injection, prepare at least 100µL of sample. For flow-injection analysis 20µL is typically sufficient. 4. Recommended sample concentrations are as follow: 1) Small organics: 10-100µM 2) Small peptides: 5 µM (pmol/µL) 3) Large proteins: 20-100µM (pmol/µL) 4) Polymers: 100-1000µM
Creative Proteomics offers you Thermo-Fisher LCQ ESI/APCI Ion Trap-based mass spectrometry services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.
For research use only, not intended for any clinical use.