Proteomics Laboratory Services
Proteomics Laboratory Services
Mass spectrometry has become invaluable in a wide range of fields and applications, including proteomics. The development of high throughput and quantitative MS proteomics workflows has expanded the scope of protein structure, function, modifications, and overall protein dynamics. In addition, MS has been widely used in metabolomics and glycomics analysis. Creative Proteomics is equipped with advanced mass spectrometers to support your experiments in academia or industry with the professional mass spectrometry-based analysis described above.
System Overview: 1. Mass range 1-400,000 Da 2. Positive and negative ion modes 3. Linear (R=3000) and reflector (R=15000) modes 4. Post-source decay (PSD) with CID 5. 337 nm nitrogen laser 6. 100 well standard sample plate (user-supplied) 7. Polymeric analysis tool (PDI, Mn, Mw, Mz calculation) 8. 5ppm mass accuracy in reflectron mode with internal reference 9. Femtomole-level detection with proper sample preparation
Applied Biosystems (formerly PerSeptive Biosystems) Voyager STR MS is a matrix assisted laser desorption ionization (MALDI) time of flight (TOF) mass spectrometer. Ions are formed in the gas phase from a laser focused onto a matrix/analyte solid sample. The matrix compound is typically a small organic molecule, chosen for its ability to absorb photons at 337 nm from the nitrogen laser. Gas phase ions (positive ions are typically protonated) are then accelerated down the 2 meter flight tube with 20-25 keV of kinetic energy (KE). Low m/z ions hit the detector first followed by the slower, higher m/z ions. To a first order, the ions follow the equation KE=1/2mv2. Typical analyte molecules include polymers, peptides, proteins, and DNA.
Sample Preparation: 1. Sample and matrix should be prepared in similar or miscible solvent systems. 2. Preparation typically only requires 1µL of sample 3. Selecting the proper matrix has a large effect on results. This decision can be based on previous successful MALDI analyses of similar samples. 4. Sinapic acid is the most common protein (> 10 kDa) matrix used. 5. α-cyano-4-hydroxycinnamic acid is the most common peptide (< 10k Da) matrix used. 6. Samples must be completely dry before loading into the instrument. 7. For positive ion samples, a small amount of acetic acid or TFA (0.1% v/v) can be added to enhance the signal. 8. Approximate recommended sample concentrations are as follow: 1) Small peptides: 1-10 pmol/µL 2) Proteins: 1-50 pmol/µL 3) Polymers: 1-10 mM
Creative Proteomics offers you Applied Biosystems Voyager DE-STR MALDI-TOF-based mass spectrometry services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.
For research use only, not intended for any clinical use.