Proteomics Laboratory Services
Proteomics Laboratory Services
Mass spectrometry has become invaluable in a wide range of fields and applications, including proteomics. The development of high throughput and quantitative MS proteomics workflows has expanded the scope of protein structure, function, modifications, and overall protein dynamics. In addition, MS has been widely used in metabolomics and glycomics analysis. Creative Proteomics is equipped with advanced mass spectrometers to support your experiments in academia or industry with the professional mass spectrometry-based analysis described above.
System Overview: 1. Mass Ranges: 15-200 Th, 50-2000 Th, 200-4000 Th 2. Resolution: 0.05 FWHM with Ultra ZoomScan 3. Sensitivity: 250 femtogram reserpine in 1:1 isopropanol:water at 400µL/min flow rate for both MS/MS and MSn. 4. ZoomScan for isotopic resolution across full-range scan 5. Ultra ZoomScan for ultimate resolution 6. Turbo Scan for ultra-fast scan to improve signal-to-noise and sampling rate 7. Positive/negative ionization modes 8. Multiple Ionization Strategies: 1) Electrospray Ionization (ESI) — Thermo Scientific Ion Max Source 2) Nanoelectrospray Ionization (nESI) — Thermo Scientific Nanospray Flex Source 3) Direct Analysis in Real Time (DART) — IonSense DART-OS Source 9. Pulsed Q Collision Induced Dissociation (PQD) enables trapping of low mass fragment ions. 10. High Resolution Isolation allows for the separation of an isobaric interfering species down to 0.3 Da or for isolation of thermally labile compounds. 11. Automatic Gain Control (AGC) used to ensure ideal number of ions stored in linear ion trap each scan. 12. MSn for n=1 to 15 for structural analysis 13. Advanced Data Dependent Experiments possible (i.e. Triple Play, Neutral Loss, Ion Tree) 14. Linear ion trap with radial ejection. Dual conversion dynode detectors used to capture high capacity of the ion signal. NanoLC Overview: 1. Pressure range: 0-1200 bar 2. Flow range: 20-2000 nL/min (Recommended: 100-1000 nL/min) 3. Samples injected via autosampler 4. Software integration with MS and instrument control using Xcalibur 4.0 5. NanoViper fitting technology enables low dead volume liquid flow path connectivity. Minimizes peak broadening due to mixing. 6. nano-ESI ionization source developed in-house utilizes a 10 µm emitter.
MANUFACTURER: Thermo Fisher Scientific
The Thermo Scientific LTQ-XL is a linear ion trap (LIT) mass spectrometer with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) capabilities. The LTQ-XL has MSn capabilities, which is a powerful analytical tool for use in structural analysis. This mass spectrometer can provide molecular weight and sequence information for small peptides (MW below 2000 Da) at the femtomole level with proper analyte introduction. Additionally, liquid seprations prior to MS detection can be made possible by coupling to the Thermo Scientific EASY-nLC 1200 liquid chromatograph with precise gradient flow (water/acetonitrile only) from 20 nL/min to 2 µL/min with pressures up to 1200 bar.
Sample Preparation: 1. All samples should be in an ESI-friendly solvent system such as 50/50 Water/Acetonitrile, Water/Methanol, etc. 2. For positive ion samples, a small amount of acetic or formic acid (0.1% v/v) can be added to enhance the signal. TFA is not recommended. 3. For direct injection, prepare at least 100µL of sample. For flow-injection analysis 20 µL is typically sufficient. 4. Recommended sample concentrations are as follow: Small organics: 1-10 µM Small peptides: 1-10 µM (pmol/µL) Large proteins: 2-10 µM (pmol/µL) Polymers: 10-100 µM
Creative Proteomics offers you Thermo Scientific LTQ-XL Linear Ion Trap Mass Spectrometer + Thermo Scientific EASY-nLC 1200 Nanoflow Liquid Chromatograph-based mass spectrometry services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.
For research use only, not intended for any clinical use.