Proteomics Laboratory Services
Proteomics Laboratory Services
Metabolomics is the science of studying chemical processes in biological systems. Metabolomic analysis based on mass spectrometry has been widely used for the qualitative and quantitative study of small molecule metabolites in different biological samples to reveal the endogenous metabolic changes occurring in cells after exogenous stimulation. Creative Proteomics offers you metabolomic analysis services based on AB SCIEX 4000 QTRAP.
This is achieved via the use of the first and third quadrupoles (Q1 and Q3, respectively) as mass filters, whilst the second quadrupole (Q2) is utilised as a collision cell in which precursor ions undergo collision-induced dissociation to produce fragment ions. The ABI 4000 QTrap is a hybrid QQQ/linear ion trap mass spectrometer, to which both 1D and 2D nano liquid chromatography (LC) separations can be interfaced. This instrument allows the array of scan types available in a QQQ setup to be exploited, with increased functionality obtained via linear ion trap mass analysis.
Quadrupole mass filters provide a robust means of sorting ions in a mass spectrometry experiment. When these mass analysers are incorporated in a triple quadrupole (QQQ) setup, which consists of three quadrupole mass filters in series, various specialised forms of quantitative and qualitative mass spectrometry can be conducted.
The system is particularly well suited to highly sensitive, targeted quantitative analyses of, for example, metabolites and complex peptide mixtures in multiple reaction monitoring (MRM) experiments. Other scan types include precursor ion scans and constant neutral loss scans, which provide highly specific means of monitoring given classes of analytes, for example classes of post-translational modifications.
Creative Proteomics offers you metabolomic analysis solutions based on AB SCIEX 4000 QTRAP. If you need our technical support, please feel free to contact us.
For research use only, not intended for any clinical use.