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Proteomics Analysis—Orbitrap Eclipse (Thermo Scientific)

Proteomics research is not only essential for exploring the mysteries of life but can also be of great benefit to human health. Proteomics research is a feature of the post-genetic era in the life sciences. Creative Proteomics can accurately identify and quantify proteins in biological systems by mass spectrometry. We are well equipped to provide Orbitrap Eclipse (Thermo Scientific)-based proteomics analysis services.

Name of the Instrument/Technicial Service

Orbitrap Eclipse (Thermo Scientific)

Introduction

One application of this instrument is multiplex quantitation of TMT-labelled samples as this instrument offers a novel method called MultiNotch MS3 combined with real time search option, which provides a higher accuracy of TMT quantification. Additionally, we use the instrument for the analysis of peptides with labile post-translational modifications such as arginine phosphorylation.

MANUFACTURER: Thermo Fisher Scientific

Summary

The Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher) includes 3 mass analyzers, i.e. a quadrupole mass filter, a linear ion trap and an Orbitrap mass analyzer and 2 detectors. The instrument allows different and partially complementary fragmentation techniques, which are collision induced dissociation (CID), higher-energy collisional dissociation (HCD), electron transfer dissociation (ETD) and a combination of ETD and HCD called EThcD.

The dedicated Creative Proteomics team can provide our clients with proteomics analysis services based on Orbitrap Eclipse (Thermo Scientific). If this is of interest to you, please feel free to contact us.

For research use only, not intended for any clinical use.

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