Proteomics Analysis—Q Exactive Plus Hybrid Quadruple-Orbitrap

Introduction

1. Superior Quantitation 1) Advanced quadrupole technology (AQT) improves precursor selection and transmission for more-accurate quantitation of low-abundance analytes in complex matrices 2) Sophisticated data-independent acquisition (DIA) and parallel reaction monitoring (PRM) deliver reproducible quantitation with complete qualitative confidence 3) An advanced active beam guide (AABG) reduces noise and extends maintenance intervals 2. Enhanced Analysis of Intact Proteins 1) Optional intact protein mode provides superior trapping of large molecules for improved analysis of intact proteins and protein complexes 2) Optional enhanced resolution ensures maximum ID confidence in top-down proteomics and lipidomics 3. High-Confidence Results 1) HR/AM and full-scan capabilities capture all sample data, all of the time, enabling retrospective data analysis without need to re-run samples 2) Resolving power up to 140,000 FWHM eliminates isobaric interferences, increasing confidence in results when analyzing samples in complex matrices 3) Better than 1ppm mass accuracy in full- and all-ion fragmentation (AIF) scan modes ensures confident compound identification 4) Fast scanning at 12Hz and spectral multiplexing suited to UHPLC applications 5) Rapid polarity switching maximizes information obtained 6) AIF and multiple dissociation techniques-in-source CID and HCD-aid in compound identification 7) Mass range to 6,000 m/z enhances detection of singly charged small molecules and biomolecules 8) More than four orders of magnitude intrascan dynamic range, along with femtogram-level sensitivity, allow detection of trace-level and high-abundance compounds in the same scan