Proteomics Analysis—Q-TOF Synapt G2 (Waters)

Summary

We use this mass spectrometer for the characterization of intact proteins. The Q-TOF Synapt G2 employs a quadrupole for the selection of precursor ions, a second quadrupole for fragmention and a time-of-flight tube for the mass analysis. The instrument is coupled to a chromatography system using a microscale C4 column, which allows chromatographic separation of proteins before being ionized in a microspray source and entering the MS. The instrument is equipped with a high-efficiency T-Wave ion mobility technology, which gives access to an additional dimension of separation, based on molecular size and shape. As Ion mobility experiments are time consuming and difficult they are not offered as a service but can be done project based. The acquisition and the operation of this instrument is based on a joint initiative with the MFPL mass spectrometry facility.