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Target Protein Characterization—Agilent HPLC for SEC in Tandem With a Wyatt MALS Detector

Creative Proteomics provides protein analytical support, from method development and fit-for-purpose validation to process/cleaning validation and sample analysis, as well as API, finished product, and impurity analysis. We specialize in a wide range of analytical assay technologies to support protein characterization and the development of recombinant proteins and peptide therapeutics.

Name of the Instrument/Technicial Service

Agilent HPLC for SEC in Tandem With a Wyatt MALS Detector

MANUFACTURER: Agilent

Summary

The Agilent HPLC 1260 can be used independently for quantitative analysis of protein samples or qualitative purification and preparation of protein samples. Additionally when used in tandem with the Wyatt DAWN Heleos-II MALS detector and an Optilab T-rEX RI detector properties of the protein sample including molecular weight and oligimerization state. The Agilent HPLC 1260 has a quaternary pump allowing for up to 4 mobile phases as well as gradients, the system has an auto-injector allowing numerous samples to be run sequentially without intervention. Also the system includes a UV-VIS detector, a fluorescent detector and a fraction collector. 1. HPLC 2. Manual 3. Wyatt MALS and RI Detectors The X-ray core laboratory has 2 HPLC columns available for use. 1. A Sepax SRT-300 for SEC separation 2. A Wyatt WTC-015S5 used exclusively for SEC-MALS experiments. Additionally users are encouraged to use additional columns specific to their experimental design.

Creative Proteomics offers an Agilent HPLC for SEC in Tandem With a Wyatt MALS Detector-based target protein characterization service that can be applied to APIs, finished products, and impurity analysis. If you are looking for technical support in this area, please contact us directly.

For research use only, not intended for any clinical use.

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