Proteomics Laboratory Services
Proteomics Laboratory Services
Mass spectrometry has become invaluable in a wide range of fields and applications, including proteomics. The development of high throughput and quantitative MS proteomics workflows has expanded the scope of protein structure, function, modifications, and overall protein dynamics. In addition, MS has been widely used in metabolomics and glycomics analysis. Creative Proteomics is equipped with advanced mass spectrometers to support your experiments in academia or industry with the professional mass spectrometry-based analysis described above.
System Overview: 1. Smartbeam laser acquisition speeds: • Max laser rep rate of 2000Hz for MS • Max laser rep rate of 1000Hz for MS/MS 2. Reflectron mode: • Resolution up to 40,000 • 1 ppm mass accuracy 3. Linear Mode: • Resolution up to ~2500 4. MS/MS or TOF/TOF mode: (LIFT mode) Mass spectra can be acquired with up to 1000 Hz laser repetition rate from low fmol levels within seconds. 5. Laser: Solid state frequency-tripled Nd : YAG laser at 355 nm with a Gaussian beam profile. 6. Digitization rate: sample rate of up to 4 GS/sec. 7. For MS Imaging work: • Laser focus diameters down to 10 microns for the best spatial resolution imaging. • Max laser rep rate of 2000Hz for MS • Max laser rep rate of 1000Hz for MS/MS
MANUFACTURER: Bruker
The Bruker UltrafleXtreme is a MALDI (Matrix-Assisted Laser Desorption Ionization) time of flight (TOF) TOF mass spectrometer. It uses delayed-extraction for linear mode, reflectron mode and MS/MS (TOF/TOF using LIFT mode) scans. Gas phase ions are formed via laser ablation and desorption of a solid chemical matrix/analyte sample, and are subsequently accelerated down a 2-meter flight tube. The flight trajectory of the ions in the tube can be modeled kinematically, therefore relating the time of flight to the mass to charge ratio (m/z) of the ion. The matrix compound is typically a small organic molecule, chosen for its ability to absorb photons at 355 nm from the frequency-tripled Nd : YAG laser. In reflectron mode the UltrafleXtreme has high mass resolving capabilities up to 40,000 and ppm mass accuracy. The UltrafleXtreme is also designed for TOF/TOF, a two stage, tandem mass spectrometry technique that can generate and record MS/MS or MS2 spectra. The incorporated LIFT device allows full fragment ion spectra to be acquired within a single scan. Typical analytes are biomolecules, nanoparticles, and synthetic polymers.
1. Sample and matrix should be prepared in similar or miscible solvent systems. 2. Preparation typically only requires 1µL of sample 3. Selecting the proper matrix has a large effect on results. This decision can be based on previous successful MALDI analyses of similar samples. • Sinapic acid is the most common protein (> 10 kDa) matrix used. • α-cyano-4-hydroxycinnamic acid is the most common peptide (< 10 kDa) matrix used. 4. Samples must be completely dry before loading into the instrument. 5. For positive ion samples, a small amount of acetic acid or TFA (0.1% v/v) can be added to enhance the signal. 6. Approximate recommended sample concentrations are as follow: • Small peptides: 1-10 pmol/µL • Proteins: 1-50 pmol/µL • Polymers: 1-10 mM
Creative Proteomics offers you Bruker Ultraflextreme MALDI TOF/TOF MS-based mass spectrometry services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.
For research use only, not intended for any clinical use.