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Mass Spectrometry Based Proteomics Analysis Service—EvoSep One UHPLC

Proteomics research is not only essential for exploring the mysteries of life but can also be of great benefit to human health. Proteomics research is a feature of the post-genetic era in the life sciences. Creative Proteomics can accurately identify and quantify proteins in biological systems by mass spectrometry. We are well equipped to provide EvoSep One UHPLC-based proteomics analysis services.

Name of the Instrument/Technicial Service

EvoSep One UHPLC

Introduction

STANDARD PROTEOMICS METHODS OPTIMIZED FOR FAST, HIGH-PERFORMANCE SEPARATION Our five standard methods cover a broad range of applications: 1. 30 samples per day: The longest method with the best overall proteome coverage. 2. 60 samples per day: The most popular method with an excellent balance of throughput and depth. 3. 100 samples per day: A great "in-between" method. 4. 200 samples per day: Excellent sequencing speed with most peptides per minute among our standard methods. 5. 300 samples per day: The highest peak capacity per minute with our fastest method for ultra-high throughput analysis.

Summary

The Evosep One is a standardized platform designed for robustness and high throughput. This is achieved by using Evotips as disposable trap columns, where samples are loaded and desalted offline. The autosampler picks up the tip and integrates elution with liquid chromatography. This removes sample handling steps and reduces injection cycle overheads. Minimal wear and tear on mechanical components is ensured as all elution and gradient formation happen at low pressure. The Evotip is left for disposal after each run, which then allows partial elution to extend column lifetime. Analytically important peptides are effectively retained, and cross-contamination reduced.

The dedicated Creative Proteomics team can provide our clients with proteomics analysis services based on EvoSep One UHPLC. If this is of interest to you, please feel free to contact us.

For research use only, not intended for any clinical use.

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