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Metabolic Assay Service—O2K from Oroboros

Name of the Instrument/Technicial Service

O2K from Oroboros

Summary

The O2K performs high resolution respirometry, perfect for small tissue samples (permeabilized or not) and isolated mitochondria. The Core owns two systems with two chambers each allowing for four simultaneous measurements to increase workflow. There are many experimental designs possible depending on each investigators needs. Example: Buffer -> Tissue Sample -> ADP -> Substrate such as Pyruvate/Malate -> Rotenone (Complex I inhibitor) -> Succinate (Substrate for Complex II) -> Oligomycin (ATP Synthase Inhibitor) -> FCCP (Uncoupler)

Our O2K from Oroboros-based metabolic assay service enables the identification and precise quantification of specific metabolites in biological systems in vitro and in vivo. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

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