Proteomics Laboratory Services
Proteomics Laboratory Services
As a function that balances the biosynthetic process, metabolic pathways are primarily responsible for maintaining cell growth and survival. The study of the targets of metabolic pathways in tumors and other metabolic diseases has become a major current interest. Through transcription and translation downstream of metabolism, the metabolites produced by the body can be key indicators for identifying abnormalities in metabolic pathways. Creative Proteomics has developed a Seahorse XFe24-based metabolic assay service.
The plate accommodates 24 individual wells, and up to 4 different test compounds can be injected separately into each well. A Cell Mito Stress Test typically injects oligomycin, FCCP, and rotenone/antimycin-A. A Glycolytic Rate Assay typically injects rotenone/antimycin-A followed by 2-deoxy-glucose.
The XF-24 analyzer simultaneously measures the Oxygen Consumption Rate (OCR) and the Extracellular Acidification Rate (ECAR) of cultured cells, isolated mitochondria, islets or small tissue samples. The instrument measures mitochondrial function in intact cells utilizing real-time bioenergetics - the rate of aerobic respiration and lactic acid production via glycolysis, respectively. The system performs label-free, non-destructive measurements, which allow the cultured cells to be used for further assays.
Our Seahorse XFe24-based metabolic assay service enables the identification and precise quantification of specific metabolites in biological systems in vitro and in vivo. If you are interested, please feel free to contact us.
For research use only, not intended for any clinical use.