Proteomics Analysis—Agilent 7000D GC Triple Quadrupole MS

Proteomics research is not only essential for exploring the mysteries of life but can also be of great benefit to human health. Proteomics research is a feature of the post-genetic era in the life sciences. Creative Proteomics can accurately identify and quantify proteins in biological systems by mass spectrometry. We are well equipped to provide Agilent 7000D GC Triple Quadrupole MS-based proteomics analysis services.

Name of the Instrument/Technicial Service

Agilent 7000D GC Triple Quadrupole MS

Introduction

Specifications 1. Mode of operation: EI standard, CI optional 2. Filaments: Dual filaments for EI 3. Electron energy: 10 to 300 eV 4. Transfer line temperature: 0 to 400℃ 5. Ion source temperature: 150 to 350℃ 6. Quadrupole temperature: 150 to 200℃ 7. Mass range: 10 to 1,050 m/z 8. Scan rate: Up to 20,000 amu/s 9. Tuning: Autotune or manual 10. MRM speed: 800 transitions/sec 11. Minimum MRM dwell: 0.5 msec 12. Collision cell: Linear hexapole 13. Collision cell gas: Nitrogen with helium quench gas 14. Collision energy: Selectable up to 60 eV 15. PAL3 Autosampler System 1) Liquid injection 2) Headspace injection 3) SPME injection 16. Oven temperature: Ambient + 4 to 450℃ 17. Oven ramps/plateaus: Supports 20 oven ramps and 21 plateaus Negative ramps are allowed 18. Ramp rate: up to 75℃/min (120 V)

MANUFACTURER: Agilent

Summary

This mass spectrometer is the newest version of the Agilent GC-MS/MS featuring both electron impact (EI) and chemical ionization (CI) techniques. The instrument is also Agilent single quadrupole compatible permitting users to load and execute old GC-MS methods. The triple quadrupole allows users to design experiments with neutral ion loss, neutral ion gain, precursor scan, product ion scan, multiple reaction monitoring (MRM), MS1 scan, and MS1 single ion monitoring (SIM). Utilizes PAL autosampler arm with liquid injection, headspace, and SPME application capabilities.

Technical Capabilities

1. Target known parent ions and daughter fragments 2. library matching of unknown compounds 3. manual liquid, headspace or SPME injections 4. match retention time and fragmentation to known standards

The dedicated Creative Proteomics team can provide our clients with proteomics analysis services based on Agilent 7000D GC Triple Quadrupole MS. If this is of interest to you, please feel free to contact us.