Proteomics Analysis—Ltq-Orbitrap Velos Pro With Etd From Thermo-Fisher Scientific With Triversa Nanomate From Advion Or Ultimate 3000 Rslcnano Hplc From Dionex

Summary

1. ESI Chip of the TriVersa consists of an array of 400 nanoelectrospray emitters with different inner diameters allowing for flow rates of 20 to 300 nL/min. 2. Nozzles on the ESI Chip provide a long, stable spray, and in infusion mode, eliminate sample-to-sample carryover. 3. TriVersa can be used for Chip-based infusion or LC/MS fraction collection 4. TriVersa can be coupled to any conventional HPLC system for MS analysis 5. Higher pressure capability of the RSLCnano platform allows the use of longer columns and/or columns packed with smaller particles for higher resolution separations of complex (digested) protein samples 6. Temperature-controlled column compartment for maintaining exceptional retention time reproducibility 7. LTQ-Orbitrap Velos Pro has resolution of 60,000 at m/z 400 at a scan rate of ¹Hz 8. Mass accuracy of < 3 ppm with external calibration 9. Equipped with HCD cell for more efficient quantitation of isobarically labeled peptides 10. Equipped with Electron Transfer Dissociation (ETD) to generate complementary information for highly sensitive PTM analyses and de novo sequencing 11. Parallel MS and MSn analysis