Crystal Characterization—Wyatt DynaPro NanoStar Dynamic Light Scattering Instrument

It is well known that no crystal is perfect. Therefore, one will always find deviations from the ideal structure in natural or artificially produced crystals. These deviations may be related to compositional inhomogeneities, impurities, or defects, all of which affect most of the physical properties of the crystal. The characterization of crystals is therefore a prerequisite for a meaningful interpretation of the results obtained. It is also important from an application point of view. Creative Proteomics offers a crystal characterization service based on Wyatt DynaPro NanoStar Dynamic Light Scattering Instrument.

Name of the Instrument/Technicial Service

Wyatt DynaPro NanoStar Dynamic Light Scattering Instrument

Introduction

1. Traditional batch dynamic light scattering The DynaPro NanoStar uniquely combines two optimized 90° detections channels: one for DLS and one for static light scattering for determining hydrodynamic Radius (Rh) and Molecular weight. The NanoStar performs robust, accurate measurements with as little as 2 µL of sample. 2. On-Line with SEC-MALS Dynamic Light Scattering Using DLS on-line with SEC-MALS will provide hydrodynamic radii and, in combination with MALS, the shape factor Rg:Rh of macromolecules > 10nm. 3. Specifications 1) Size Range, Rh, by DLS: 0.2 to 25000 nm 2) Molar Mass Range by SLS: 300 Da to 1 MDa 3) Sensitivity (lysozyme 14. 4 kDa): 0.1 mg/mL 4) Minimum Sample Volume: 2 µL (quartz cuvette),4 µL (disposable cuvette) 5) Laser Wavelength: 658 nm 6) Laser Power Control: Programmable 10% to 100% 7) Temperature Control: -15 to +120℃

Summary

The DynaPro NanoStar can be operated using microcuvette-based format to analyze size distributions and particle concentrations of proteins, nanomedicines, viruses, vesicles, emulsions, micelles and metallic nanoparticles.

Applications

1. Hydrodynamic Radius (Rh) 2. Intensity, Mass and Number distributions 3. Aggregation 4. Molecular Weight 5. A2-Second Virial Coefficient 6. Temperature transition (Tonset)

Creative Proteomics offers crystal characterization services to help you understand the cell parameters, dot patterns, atomic coordinates of simple structures, and much more. If you are interested, please contact us directly.