Proteomics Laboratory Services
Proteomics Laboratory Services
Mass spectrometry has become invaluable in a wide range of fields and applications, including proteomics. The development of high throughput and quantitative MS proteomics workflows has expanded the scope of protein structure, function, modifications, and overall protein dynamics. In addition, MS has been widely used in metabolomics and glycomics analysis. Creative Proteomics is equipped with advanced mass spectrometers to support your experiments in academia or industry with the professional mass spectrometry-based analysis described above.
System Overview: 1. Mass Ranges: 50-6,000 Th (standard mode), 350-20,000 Th (EMR mode) 2. Resolution: Up to 140,000 (FWHM) @ m/z 200 3. Mass Accuracy: 1) Standard mode: < 1 ppm RMS (internal) ; < 3 ppm RMS (external) 2) EMR mode (external) : < 3 ppm RMS (350-6,000 Th) ; < 5 ppm RMS (6,001-10,000 Th) ; < 10 ppm RMS (10,001-15,000 Th) 4. Sensitivity: 500 fg buspirone on-column S/N 100:1 acquired with H-ESI II probe 5. Max scan rate: Up to 12Hz at mass resolution setting of 17,500 @ m/z 200 6. Positive/negative ionization modes 7. All-ion-fragmentation (AIF) with either: 8. In-source collision-induced dissociation (CID) 9. Higher-energy collisional dissociation (HCD) 10. Multiple Ionization Strategies: 11. Electrospray Ionization (ESI) — Thermo Scientific Ion Max Source 12. Nanoelectrospray Ionization (nESI) — Thermo Scientific Nanospray Flex Source 13. Direct Analysis in Real Time (DART) — IonSense DART-OS Source UHPLC Overview: 1. Pressure range: 290-15,000 psi 2. Flow range: 0.001-8 mL/min 3. Samples injected via autosampler 4. Software integration with MS and instrument control using Xcalibur 4.0 5. NanoViper fitting technology enables low dead volume liquid flow path connectivity. Minimizes peak broadening due to mixing.
MANUFACTURER: Thermo Fisher Scientific
The Thermo Scientific Exactive Plus EMR is a Orbitrap-based electrostatic ion trap mass spectrometer with electrospray (ESI), nanoelectrospray (nESI), and direct analysis in real time (DART) ionization capabilities. The Exactive Plus EMR has high resolution and accurate mass (HRAM) capabilities, providing high confidence molecular weight determinations. This mass spectrometer can provide molecular weight information for small molecules at the femtomole level with proper analyte introduction. Additionally, liquid seprations prior to MS detection can be made possible by coupling to the Thermo Scientific Vanquish Flex Binary UHPLC with precise gradient flow from 0.001-8 mL/min with pressures up to 15,000 psi.
Sample Preparation: 1. All samples should be in an ESI-friendly solvent system such as 50/50 Water/Acetonitrile, Water/Methanol, etc. 2. For positive ion samples, a small amount of acetic or formic acid (0.1% v/v) can be added to enhance the signal. TFA is not recommended. 3. For direct injection, prepare at least 100µL of sample. For flow-injection analysis 20µL is typically sufficient. 4. Recommended sample concentrations are as follow: 1) Small organics: 1-10 µM 2) Small peptides: 1-10 µM (pmol/µL) 3) Large proteins: 2-10 µM (pmol/µL) 4) Polymers: 10-100 µM
Creative Proteomics offers you Thermo Scientific Exactive Plus EMR Orbitrap Mass Spectrometer + Thermo Scientific Vanquish Flex Binary UHPLC-based mass spectrometry services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.
For research use only, not intended for any clinical use.